Adipose-derived stem cells

methods and protocols by Jeffrey M. Gimble

Publisher: Humana Press in New York, NY

Written in English
Published: Pages: 473 Downloads: 581
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Subjects:

  • Cultured Cells,
  • Cytology,
  • Stem Cells,
  • Cell Culture Techniques,
  • Laboratory Manuals,
  • Adipose Tissue,
  • Stem cells,
  • Adipose tissues

Edition Notes

Includes bibliographical references and index.

Statementedited by Jeffrey M. Gimble, Bruce A. Bunnell
SeriesSpringer protocols, Methods in molecular biology -- 702
Classifications
LC ClassificationsQH588.S83 A325 2011
The Physical Object
Paginationxv, 473 p. :
Number of Pages473
ID Numbers
Open LibraryOL25095178M
ISBN 10161737959X
ISBN 109781617379598, 9781617379604
LC Control Number2011283265
OCLC/WorldCa663950237

Natural extracellular matrices (ECMs) have been widely used as a support for the adhesion, migration, differentiation, and proliferation of adipose-derived stem cells (ADSCs). However, poor mechanical behavior and unpredictable biodegradation properties of natural ECMs considerably limit their potential for. Given the various attributes of stem cell therapies using Adipose-Derived(ASDC) cells, it is easy to see that they are highly relevant for clinical and therapeutic applications. Learn more about the advantages of ASDC stem cells and their place in Regenerative Medicine. Isolation and culture of rhesus adipose-derived stem cells / Christine Gagliardi and Bruce A. Bunnell --Isolation of human adipose-derived stem cells from lipoaspirates / Gang Yu [and others] --Isolation of murine adipose-derived stem cells / Gang Yu [and others] --In vitro adult rat adipose tissue-derived stromal cell isolation and. Title:The Basic Mechanism of Hair Growth Stimulation by Adipose-derived Stem Cells and Their Secretory Factors VOLUME: 12 ISSUE: 7 Author(s):Chong Hyun Won*, Gyeong-Hun Park, Xunwei Wu, Thanh-Nga Tran, Ki-young Park, Byung-Soon Park, Dong Young Kim, Ohsang Kwon and Kyu-Han Kim* Affiliation:Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Cited by: 7.

Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro including adipocytes, osteoblasts, and chondrocytes (Figure 1).In vitro differentiation into non-mesenchymal cell types, such as neuronal and glial progenitors, hepatocytes and vascular endothelial progenitors, cardiomyocytic, pancreatic and epithelial phenotypes has also been described. This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC.   Stem cell therapy is a promising strategy for the treatment of severe cerebral ischemia. However, targeting sufficient grafted cells to the affected area remains challenging. Choosing an adequate transplantation method for the CNS appears crucial for this therapy to become a clinical by: 3.

Adipose-derived stem cells by Jeffrey M. Gimble Download PDF EPUB FB2

Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.

From the reviews: “This book is pleasing the reader. Clear enough, the statistics is impressive. I am Adipose-derived stem cells book sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will for sure appear.

Adipose-derived stem cells. Human adipose-derived stem cells (hADSC) can be easily and safely retrieved from relatively small volumes of fat in adults and children. Adipose-derived stem cells book cells can be cultured relatively easily and are capable of self-renewal and possess multiline age.

The regenerative cells that can be isolated from subcutaneous fat have been designated adipose-derived stem or stromal cells, ADRCs, adipose-derived adult stem cells, adipose-derived adult stromal cells, adipose-derived stromal cells, adipose mesenchymal stem cells, lipoblasts, pericytes, preadipocytes, vascular stromal fraction, and processed Author: WA Gathier, Z Türktas, HJ Duckers.

In this study we have shown that human adipose-derived stem cells (ASCs) and bone marrow mesenchymal stem cells (BMSCs) populations display a common expression profile. Isolation of Human Adipose-Derived Stem Cells from LipoaspiratesJie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C.

Halvorsen, and Jeffrey M. Gimble Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method Jie Li, Hui Li, and Weidong Tian In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells.

Human adipose-derived stem cells (hASCs) differentiate in vitro to adipocytes, cardiac myocytes, smooth muscle, osteocytes, and chondrocytes when treated with specific factors. Most people know only of this controversial method of harvesting stem cells but, fortunately, we now know that adult stem cells can be harvested and used in therapies.

With easy access to adult human adipose-derived stem cells and bone marrow, there is no need to deal with embryonic cells any longer. Functional Roles of Adipose Tissue-Derived Stem Cells in Regenerative Medicine. The beneficial effects of stem cells and therewith the paradigm of tissue regeneration may not be restricted to cellular restoration but may also be related to the transient paracrine actions of the cells [].In Roh et al.

[] reported that human-derived MSCs seeded on polymer scaffolds were no longer detectable Cited by: The process is much easier with placental-derived stem cells, as no harvesting is required. But let’s look closer into each type of stem cell and see how they share similarities or differ: Adipose Derived Stem Cells.

Adipose/fat tissue is a great source of stem cells and growth factors for patients of all ages. Adipose Derived Stem Cells: Current State of the Art and Prospective Role in Regenerative Medicine and Tissue Engineering. By Vincenzo Vindigni, Giorgio Giatsidis, Francesco Reho, Erica Dalla Venezia, Marco Mammana and Bassetto Franco.

Submitted: May 12th Reviewed: January 22nd Published: May 22nd DOI: /Cited by: 2. De Villiers J, Houreld N and Abrahamse H Influence of low intensity laser irradiation on isolated human adipose derived stem cells over 72 hrs and their differentiation potential into smooth muscle cells using retinoic acid Stem Cell Rev.

Rep. 7 – Crossref. From the reviews: "This book is pleasing the reader. Clear enough, the statistics is impressive. I am quite sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will for sure appear more.

Louis, MO, USA – Using induced pluripotent stem cells produced from the skin of a patient with a rare, genetic form of insulin-dependent diabetes called Wolfram syndrome, researchers transformed the human stem cells into insulin-producing cells and used the gene-editing tool CRISPR-Cas9 to correct a genetic defect that had caused the syndrome.

Adipose-derived stem cells are multipotent, undifferentiated, and self-renewing. They are morphologically and phenotypically similar to other MSCs. Because obtaining adipose cells is a minimally invasive procedure, and the proliferation capacity does not decline with age, they have been proven superior to bone marrow-derived stem cells by many.

Cells, an international, peer-reviewed Open Access journal. Dear Colleagues, Adipose tissue is a rich, ubiquitous and easily accessible source for multipotent stromal/stem cells. Adipose-derived Stem Cells in Tissue Regeneration: A Review.

ISRN Stem Cells. in press. Zuk PA, et al. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell. ; – [PMC free article] [Google Scholar] Cited by: Adipose-Derived Stem Cells (ASCs) for Tissue Engineering. By Mathias Tremp, Souzan Salemi, Rita Gobet, Tullio Sulser and Daniel Eberli.

Submitted: November 17th Reviewed: April 19th Published: August 29th DOI: /Cited by: In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells.

With chapters organized around 1/5(1). Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily.

Three-dimensional (3D) tissue engineering scaffolds are better able to mimic the in vivo cellular microenvironment, which benefits the Cited by: Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.

Product details. Series: Methods in Molecular Biology (Book )Format: Hardcover. Mesenchymal stem/stromal cells (MSCs) have immunosuppressive and regenerative properties. Adipose tissue is an alternative source of MSCs, named adipose-derived mesenchymal stem cells (ASCs).

Because the biology of ASCs in rheumatic diseases (RD) is poorly understood, we performed a basic characterization of RD/ASCs. The phenotype and expression of adhesion molecules (intracellular Author: Ewa Kuca-Warnawin, Urszula Skalska, Iwona Janicka, Urszula Musiałowicz, Krzysztof Bonek, Piotr Głusz.

Editorial Reviews. From the reviews: “This book is pleasing the reader. Clear enough, the statistics is impressive. I am quite sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will Brand: Jeffrey M.

Gimble. adipose stem cells: A population of cells within adipose tissue which retain a high proliferation capacity in vitro and the ability to undergo extensive differentiation, and thus are biologically similar to mesenchymal stem cells derived from the bone marrow. Differentiation results in stem cells adopting the phenotypic, biochemical and.

Inresearchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative by: Differential circular RNA expression profiling during osteogenic differentiation in human adipose-derived stem cells.

Kang, Y., Guo, S., ‘Primitive’ Stem Cells Shown to Regenerate Blood Vessels in The Eye. Abnormal Bone Formation After Trauma Explained and Reversed in Mice. Human adipose-derived stem cells: current challenges and clinical perspectives the replicating function of stem cells.

13 The stromal vascular fraction (SVF) of adipose tissue has been the focus of recent research on adi-pose-derived stem cells – ADSC. 9,11,14,15 Some studies indicate that this tissue compartment is a rich source.

Adipose-derived regenerative cells (ADRC) consists of several cell types, such as adult stem cells, vascular endothelial cells, and vascular smooth muscle cells, among others.

These cells contribute to wound repair through a variety of mechanisms by promoting blood vessel growth and blocking apoptosis. Moreover, adipose-derived mesenchymal stem cells secrete a wide range of growth factors that can stimulate tissue regeneration. However, the potential of adipose-derived mesenchymal stem cells differs depending on the donor’s medical condition, including diseases such as diabetes.

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery.

In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established.Adipose-derived Stem Cell Markers Adipose tissue is composed primarily of adipocytes, cells that function to store energy in the form of lipid droplets.

Additionally, pluripotent progenitor cells, called adipose-derived stem cells, can be identified in adipose tissue by the expression of markers such as Integrin family members, CD44, and ICAMChapter 6 – Adipose Derived Stem Cells.

Adipose tissue in adult humans contains non-pluripotent Stem Cells that are significantly easier to harvest than other types of Stem Cell, and which show a greater effectivity, as they are used in the patient’s own treatment. This lecture covers the science behind Stem Cells found in adipose tissue.